Scientist II

Job Title: Scientist II

Duties:

• Plan, coordinate and perform high throughput expression of biotherapeutic proteins using suspension CHO cells at various scales (plate based, tube based, and flask based) with automated platforms; Determine key cell-culture parameters and interpret findings.

• Transfection, Culture monitoring, Harvest, Titer determination and Western blot analysis as needed.

• Design and produce DNA for use in transient and stable transfections. Perform molecular biology techniques as necessary: cloning, DNA scale up, sequencing analysis

• Evaluate new technologies and process changes to improve the productivity, quality and consistency of the expression processes.

• Independently interpret, communicate and present results through reports and internal presentations.

• Work closely with counterparts from other functional areas to deliver research milestones while adhering to broad project timelines.

• Comply with all applicable regulations, perform all work in a safe manner; maintain proper records in accordance with SOP's and policies.

• Contributes to lab organization, reagent tracking and other tasks as determined appropriate

Skills:

• Thorough understanding of cell culture basics.

• Hands-on experience in cell culture (mammalian) and exceptional aseptic techniques.

• Strong attention to details and excellent organizational skills.

• Able to learn quickly and excel in a past-faced work environment, self-motivation, adaptability and positive attitude.

• Hands-on experience in handling automation/HTP antibody expression is a plus

• Ability to work in plate-based formats (24 well, 96 well)

• Knowledge of DNA and molecular biology including basic DNA quality assessments

• Apply knowledge of basic theory and scientific principles

• Concise and accurate reporting of technical data and information

• Ability to troubleshoot and multi-task

• Ability to work in a team-oriented environment

• Excellent written and verbal communication skills

Education:

• Bachelor's Degree from an accredited institution with three-plus (3+) years of experience in related scientific discipline, or master’s degree from an accredited institution in related scientific discipline or equivalent experience in related field